Microbiology

Culture & Microbiology Services

Conventional culture, microscopy, isolation and identification of bacterial and fungal pathogens — the foundation for susceptibility testing.

Method
Culture, microscopy, biochemical identification
Turnaround
Data to be updated
Specimen
Whole Blood (EDTA), Sputum, Urine, Stool, Tissue / Biopsy, Pus / Aspirate
Sample volume
Data to be updated

Why This Test Matters

Culture remains irreplaceable: it recovers viable organisms, proves they are alive, enables colony-based identification and — critically — provides the isolate without which phenotypic antimicrobial susceptibility testing is impossible.

Clinical Background

Microbiology is the study of microorganisms — bacteria, viruses, fungi and protozoa — their morphology, physiology, pathogenesis and clinical significance. Culture is its oldest and still most versatile tool.

The Diagnostic Challenge

Current Diagnostic Methods

Microscopy (Gram, AFB stains), inoculation onto selective and enriched media, incubation, colony morphology assessment, and biochemical identification form the classical pathway.

How the ID Labs Test Helps

ID Labs performs bacterial, fungal and mycobacterial culture with isolation and identification, providing the viable organisms required for antimicrobial susceptibility testing and for correlation with molecular results.

Test Methodology

MethodologyCulture, microscopy, biochemical identification
TechnologyConventional microbiology
InstrumentData to be updated
DetectionData to be updated

Specimen Information

Whole Blood (EDTA)

Collection
Venepuncture into EDTA tube using aseptic technique.
Minimum volume
2 mL
Recommended
3–5 mL
Transport
2–8 °C, do not freeze
Storage
2–8 °C
Stability
Data to be updated
Rejection criteria
Clotted, haemolysed or unlabelled samples; wrong anticoagulant.

Sputum

Collection
Deep cough specimen into a sterile wide-mouth container after rinsing the mouth.
Minimum volume
1 mL
Recommended
3–5 mL
Transport
2–8 °C
Storage
2–8 °C
Stability
Data to be updated
Rejection criteria
Saliva-only samples (watery, no purulent material).

Urine

Collection
Clean-catch midstream urine into a sterile container.
Minimum volume
1 mL
Recommended
5–10 mL
Transport
2–8 °C
Storage
2–8 °C
Stability
Data to be updated
Rejection criteria
Unpreserved urine held at room temperature > 2 hours.

Stool

Collection
Fresh stool in a clean, leak-proof container.
Minimum volume
1 g / 1 mL
Recommended
5 g
Transport
2–8 °C
Storage
2–8 °C
Stability
Data to be updated
Rejection criteria
Dried-out or contaminated samples.

Tissue / Biopsy

Collection
Aseptically collected tissue in sterile container with a small amount of sterile saline.
Minimum volume
Data to be updated
Recommended
Data to be updated
Transport
2–8 °C
Storage
2–8 °C
Stability
Data to be updated
Rejection criteria
Formalin-fixed tissue is unsuitable for culture and most molecular assays.

Pus / Aspirate

Collection
Aspirated pus in sterile container or swab in transport medium.
Minimum volume
0.5 mL
Recommended
1–2 mL
Transport
2–8 °C
Storage
2–8 °C
Stability
Data to be updated
Rejection criteria
Superficial swabs contaminated with skin flora.

Specimen Container

Sterile Screw-Cap Container

Universal sterile container · Cap: White

Additive: None

Volume: 10–30 mL capacity

Collect aseptically; label immediately.

Blood Culture Bottle (Aerobic/Anaerobic)

Culture bottle · Cap: Varies by system

Additive: Enriched broth with anticoagulant (SPS)

Volume: 8–10 mL blood per bottle (adult)

Disinfect skin and bottle septum; inoculate before any other tube.

Urine Container (Sterile, Wide-mouth)

Sterile container · Cap: Yellow/White

Additive: None (boric acid versions available)

Volume: 5–10 mL

Midstream clean-catch sample preferred.

Sputum Container (Wide-mouth, Sterile)

Sterile container · Cap: White

Additive: None

Volume: 3–5 mL

Collect early-morning deep-cough sample for mycobacterial testing.

Targets / Analytes / Organisms

Validated target list: data to be updated. Please contact ID Labs for the current configuration.

Technical Performance

CharacteristicValueSource
Analytical / clinical performancePerformance characteristics: please contact ID Labs — data to be updated.ID Labs Validated Performance

Clinical Utilization

Advantages

  • Recovers viable organisms
  • Enables phenotypic AST
  • Detects organisms not covered by any panel
  • Supports epidemiology and outbreak work

Limitations

  • Slow — 24–72 hours for common bacteria, weeks for mycobacteria
  • Low sensitivity for fastidious organisms and after antibiotics
  • Transport conditions determine viability
  • Molecular detection does not automatically replace culture: the two answer different questions

Publications & Scientific Evidence

Automated blood-culture systems (BacT/ALERT VIRTUO vs BACTEC FX400): comparative evaluation

Microbiology Spectrum (ASM) · 2024

Comparative StudyExternal link only

Curated peer-reviewed / authoritative reference. Open the source link for the full text.

MALDI-TOF Biotyper for bacterial identification: multicenter accuracy study

PLOS ONE · 2015

Clinical ValidationExternal link only

Curated peer-reviewed / authoritative reference. Open the source link for the full text.

Discuss this test with our scientific team

Panel configuration, specimen logistics and turnaround details on request.

Enquire now

Information on this page is educational and does not replace professional clinical judgment. Performance values are labelled by source (ID Labs Specification / ID Labs Validated Performance / Manufacturer Specification / Published Scientific Evidence). Molecular results must be correlated clinically and, where appropriate, confirmed by culture and phenotypic susceptibility testing.