CLIA/ECLIA versus ELISA for infectious-disease serology screening: comparative performance
Indian Journal of Clinical Biochemistry (PMC) · 2019
Curated peer-reviewed / authoritative reference. Open the source link for the full text.
Rapid, non-invasive detection of antibodies or antigens to support diagnosis, staging and immune-status assessment of infectious diseases.
Serology detects the immune response to infection — or the pathogen's antigens directly — enabling diagnosis of diseases that are difficult to culture or amplify, screening of blood donations, epidemiological surveillance and vaccine-response assessment.
When the immune system meets a pathogen it produces antibodies: IgM appears early (recent/acute infection), followed by IgG (past exposure, chronic infection or immunity); IgA contributes at mucosal sites. Antigen tests instead detect components of the pathogen itself, indicating active infection.
ELISA is the workhorse for detecting antibodies or antigens with high sensitivity and specificity. Western blot confirms positive screening results (classically for HIV). Rapid tests deliver point-of-care answers. Immunofluorescence assays detect antibodies using fluorescence microscopy.
ID Labs offers rapid, non-invasive serological testing that aids early diagnosis, monitoring of infection progression and assessment of immune status across viral, bacterial, fungal and parasitic infections.
Evacuated blood tube · Cap: Red / Gold
Additive: None / clot activator with gel
Volume: 5 mL
Allow to clot 30 minutes, centrifuge, separate serum.
Validated target list: data to be updated. Please contact ID Labs for the current configuration.
| Characteristic | Value | Source |
|---|---|---|
| Analytical / clinical performance | Performance characteristics: please contact ID Labs — data to be updated. | ID Labs Validated Performance |
Diagnosis (e.g., HIV, hepatitis, syphilis, dengue serology), blood-donor screening, epidemiological studies and vaccine-efficacy assessment. Interpretation must consider the window period — the interval before antibodies become detectable.
| PCR vs Serology | ||
|---|---|---|
| Feature | PCR (molecular) | Serology |
| Detects | Pathogen nucleic acid | Host antibodies / pathogen antigens |
| Best timing | Acute phase | After window period; staging |
| Past infection / immunity | No | Yes (IgG) |
Indian Journal of Clinical Biochemistry (PMC) · 2019
Curated peer-reviewed / authoritative reference. Open the source link for the full text.
LSHTM Research Online · 2025
Curated peer-reviewed / authoritative reference. Open the source link for the full text.
Panel configuration, specimen logistics and turnaround details on request.
Information on this page is educational and does not replace professional clinical judgment. Performance values are labelled by source (ID Labs Specification / ID Labs Validated Performance / Manufacturer Specification / Published Scientific Evidence). Molecular results must be correlated clinically and, where appropriate, confirmed by culture and phenotypic susceptibility testing.