Infectious Serology

Infectious Serology

Rapid, non-invasive detection of antibodies or antigens to support diagnosis, staging and immune-status assessment of infectious diseases.

Method
ELISA, rapid tests, immunofluorescence, Western blot
Turnaround
Data to be updated
Specimen
Serum, Plasma
Sample volume
Data to be updated

Why This Test Matters

Serology detects the immune response to infection — or the pathogen's antigens directly — enabling diagnosis of diseases that are difficult to culture or amplify, screening of blood donations, epidemiological surveillance and vaccine-response assessment.

Clinical Background

When the immune system meets a pathogen it produces antibodies: IgM appears early (recent/acute infection), followed by IgG (past exposure, chronic infection or immunity); IgA contributes at mucosal sites. Antigen tests instead detect components of the pathogen itself, indicating active infection.

The Diagnostic Challenge

Current Diagnostic Methods

ELISA is the workhorse for detecting antibodies or antigens with high sensitivity and specificity. Western blot confirms positive screening results (classically for HIV). Rapid tests deliver point-of-care answers. Immunofluorescence assays detect antibodies using fluorescence microscopy.

How the ID Labs Test Helps

ID Labs offers rapid, non-invasive serological testing that aids early diagnosis, monitoring of infection progression and assessment of immune status across viral, bacterial, fungal and parasitic infections.

Test Methodology

MethodologyELISA, rapid tests, immunofluorescence, Western blot
TechnologyImmunoassay
InstrumentData to be updated
DetectionData to be updated

Specimen Information

Serum

Collection
Venepuncture into plain/serum-separator tube; allow clotting and centrifuge.
Minimum volume
0.5 mL
Recommended
1–2 mL
Transport
2–8 °C
Storage
2–8 °C short term; −20 °C for longer storage
Stability
Data to be updated
Rejection criteria
Gross haemolysis, lipaemia, insufficient volume.

Plasma

Collection
Venepuncture into EDTA/heparin tube; centrifuge and separate plasma.
Minimum volume
0.5 mL
Recommended
1–2 mL
Transport
2–8 °C
Storage
2–8 °C; freeze for long term
Stability
Data to be updated
Rejection criteria
Haemolysed samples; delayed separation.

Specimen Container

Plain / Serum Separator Tube

Evacuated blood tube · Cap: Red / Gold

Additive: None / clot activator with gel

Volume: 5 mL

Allow to clot 30 minutes, centrifuge, separate serum.

Targets / Analytes / Organisms

Validated target list: data to be updated. Please contact ID Labs for the current configuration.

Technical Performance

CharacteristicValueSource
Analytical / clinical performancePerformance characteristics: please contact ID Labs — data to be updated.ID Labs Validated Performance

Clinical Utilization

Diagnosis (e.g., HIV, hepatitis, syphilis, dengue serology), blood-donor screening, epidemiological studies and vaccine-efficacy assessment. Interpretation must consider the window period — the interval before antibodies become detectable.

Advantages

  • Non-invasive (serum/plasma)
  • Establishes stage of infection (IgM vs IgG)
  • High-throughput and cost-effective
  • Detects past infection and immunity, which PCR cannot

Limitations

  • Window period: early infection can be antibody-negative
  • Seroconversion takes days to weeks — serology has limited value in the first days of acute illness
  • Cross-reactivity can cause false positives
  • A positive IgG indicates exposure, not necessarily active disease
  • PCR and serology answer different questions and are often complementary

Conventional vs Advanced Technology

PCR vs Serology
FeaturePCR (molecular)Serology
DetectsPathogen nucleic acidHost antibodies / pathogen antigens
Best timingAcute phaseAfter window period; staging
Past infection / immunityNoYes (IgG)

Publications & Scientific Evidence

CLIA/ECLIA versus ELISA for infectious-disease serology screening: comparative performance

Indian Journal of Clinical Biochemistry (PMC) · 2019

Comparative StudyExternal link only

Curated peer-reviewed / authoritative reference. Open the source link for the full text.

Evaluating the performance of common dengue serological assays (NS1/IgM)

LSHTM Research Online · 2025

Clinical StudyExternal link only

Curated peer-reviewed / authoritative reference. Open the source link for the full text.

Frequently Asked Questions

Discuss this test with our scientific team

Panel configuration, specimen logistics and turnaround details on request.

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Information on this page is educational and does not replace professional clinical judgment. Performance values are labelled by source (ID Labs Specification / ID Labs Validated Performance / Manufacturer Specification / Published Scientific Evidence). Molecular results must be correlated clinically and, where appropriate, confirmed by culture and phenotypic susceptibility testing.