Multiplex real-time PCR for SARS-CoV-2, influenza A/B and RSV: analytical and clinical evaluation
Journal of Clinical Virology (PMC) · 2021
Curated peer-reviewed / authoritative reference. Open the source link for the full text.
Comprehensive viral and bacterial respiratory pathogen detection from swabs, sputum or BAL in a single multiplex assay.
Respiratory infections — from upper respiratory illness to radiologically confirmed pneumonia and bronchiolitis — represent approximately half of consultations in some primary-care datasets and are among the most common reasons for hospital admission.
Viral and bacterial respiratory infections present with overlapping features but demand different management: antivirals and isolation for some viruses, antibiotics for bacterial pneumonia, and no antibiotics for most viral illness. Distinguishing them clinically is unreliable.
Rapid antigen tests are fast but insensitive; culture takes days and detects only bacteria; single-plex PCRs require multiple sequential tests and samples.
Clinical diagnosis, chest imaging, rapid antigen tests, sputum culture and single-target PCRs form the conventional pathway.
A single multiplex RT-PCR tests one specimen against the common viral and bacterial respiratory pathogens simultaneously, delivering a comprehensive aetiological picture in one run.
Universal sterile container · Cap: White
Additive: None
Volume: 10–30 mL capacity
Collect aseptically; label immediately.
Swab transport tube · Cap: Varies
Additive: Viral transport medium
Volume: 2–3 mL VTM
Break swab shaft into medium; cap tightly.
Sterile container · Cap: White
Additive: None
Volume: 3–5 mL
Collect early-morning deep-cough sample for mycobacterial testing.
| Characteristic | Value | Source |
|---|---|---|
| Analytical / clinical performance | Performance characteristics: please contact ID Labs — data to be updated. | ID Labs Validated Performance |
Consider in moderate-to-severe respiratory illness, hospitalised pneumonia, bronchiolitis in infants, immunocompromised patients, and during influenza/COVID-19 season for triage and isolation decisions.
Journal of Clinical Virology (PMC) · 2021
Curated peer-reviewed / authoritative reference. Open the source link for the full text.
Frontiers in Medicine · 2023
Curated peer-reviewed / authoritative reference. Open the source link for the full text.
Rapid molecular investigation of multiple pathogens associated with meningitis and encephalitis from a single CSF sample.
Rapid detection of more than 30 sepsis-causing pathogens and their drug-resistance markers directly from whole blood.
Simultaneous detection of viruses, parasites and bacteria causing acute febrile illness and pyrexia of unknown origin from one sample.
Broad molecular screening for opportunistic viral, bacterial and fungal pathogens in transplant and immunocompromised patients.
Rapid syndromic testing tailored to febrile and seriously ill children, designed around small sample volumes.
Molecular detection of common sexually transmitted pathogens from a single urogenital specimen.
Panel configuration, specimen logistics and turnaround details on request.
Information on this page is educational and does not replace professional clinical judgment. Performance values are labelled by source (ID Labs Specification / ID Labs Validated Performance / Manufacturer Specification / Published Scientific Evidence). Molecular results must be correlated clinically and, where appropriate, confirmed by culture and phenotypic susceptibility testing.